PAPER TITLE :ANTIBACTERIAL ACTIVITIES OF MORINGA OLEIFERA (LAM) ON COLIFORMS ISOLATED FROM SOME SURFACE WATERS IN AKURE, NIGERIA

FUTA JOURNAL OF RESEARCH IN SCIENCE | VOLUME 9 NUMBER 1 2013

Paper Details

  • Author(s) : 1 O.O. Makanjuola, E.O. Dada and F.O. Ekundayo
  • Abstract:

Antibacterial activities of Moringa oleifera (LAM) leaf extracts were carried out on coliforms isolated from surface
waters using growth inhibition indices based on agar plate technique. Hot water and two organic solvents, namely
acetone and ethanol were used for the extraction with 16.67%, 17.86% and 21.62% recovery respectively. The plant
extract was also subjected to column and thin layer chromatography for purification and fractionation of the
biologically active components. The mean count of coliforms/ml in water samples ranged from 12.80×103 cfu/ml to
48.60×103 cfu/ml. At 12.5 mg/ml, the active constituents demonstrated higher activity with 11.5 mm zone than crude
extracts (7.01 mm, 6.38 mm and 0.0 mm zones in acetone, ethanol and hot water respectively) on Escherichia coli.
Gentamycin, the reference antibiotic, had between 2.33 and 24.67 mm inhibitory zones on the isolated organisms. The
minimum inhibitory concentration ranged from 4.0 mg/ml to 35.0 mg/ml, while the minimum bactericidal concentration
ranged from 5.0 mg/ml to 45.0 mg/ml. The antibacterial activities examined in this study showed that Moringa oleifera
leaves are capable of exerting inhibitory effect on the target organisms.
Keywords: Moringa oleifera, antibacterial, surface waters and coliforms.