PAPER TITLE :INFLUENCE OF MACROPHAGES IN CONFERRING IMMUNITY AGAINST ESCHERICHIA COLI O157:H7 INFECTION IN ALBINO RATS

FUTA JOURNAL OF RESEARCH IN SCIENCE | VOLUME 9 NUMBER 1 2013

Paper Details

  • Author(s) : *B. O. Oladejo and T. T. Adebolu
  • Abstract:

The influence of macrophages in conferring immunity against Escherichia coli O157:H7 infection in albino rats was
studied by monitoring the number of organisms that were trapped in the liver, spleen, and ileum of the rats that were
administered carrageenan, which has a selective cytopathic effect on macrophages. At different intervals, starting
from 30 minutes to 168 hours, the selected organs were dissected out and the number of trapped E. coli O157:H7
was counted using standard microbiological techniques. The number of trapped E. coli O157:H7 in the spleen, liver
and ileum of carrageenan treated rats (5.66, 5.58 and 5.50 log10 cfu/ml respectively) was observed to be significantly
greater (P≤0.05) than the number that was trapped in the spleen, liver and ileum of those that were not administered
with carrageenan prior to infection (5.26, 5.28 and 5.37 log10 cfu/ml respectively) at the end of 168 hours. The
highest number of E. coli O157:H7 (5.77 log10 cfu/ml) was however recovered from the spleen of carrageenan
treated rats after 96 hours of infection. From this investigation, the administration of carrageenan resulted in high
recovery of E. coli O157:H7 from the organs of infected rats. Thus, macrophages played a significant role in
fighting against this organism in the host’s immune system.
Keywords: Macrophages, Immunity, E. coli O157:H7, Liver, Spleen, Ileum, Cytopathy