Clonal propagation study was carried out on Solanecio biafrae; a threatened indigenous leafy vegetable, using nodal and leaf explants. The aim of this study was to identify a suitable explant and determine an optimal hormonal concentration for callus induction and shoot proliferation. Nodal and leaf explants of this species were cultured on Murashige and Skoog (MS) medium, supplemented with various concentrations of Benzylaminopurine (BA) or Naphtalene acetic acid (NAA) alone and in combination. Nodal explants were found to be suitable for shoot and root proliferation and callus formation. The highest shoot regeneration frequency (100%) was obtained from nodal explants cultured on MS medium supplemented with a combination of 1.25 mg/L BA and 0.25 mg/L NAA. The highest root induction frequency (80%) was obtained from leaf explants cultured on MS supplemented with 0.50 mg/L BA. Multiple shoots and plantlets were produced from nodal explants cultured on MS medium supplemented with 1.25 mg/L BA and 0.25 mg/L NAA. The highest callus induction frequency (100%) was obtained from nodal explants cultured on MS medium supplemented with a combination of 0.25 mg/L BA and 0.50 mg/L NAA. A suitable explant for callus formation and organogenesis has been identified. This report is the first on in vitro propagation of this threatened indigenous leafy vegetable using leaf explants.
FUTA JOURNAL OF RESEARCH IN SCIENCE
VOLUME 12 NUMBER 2 2016
- Title :EFFECT OF NAPHTALENE ACETIC ACID AND BENZYLAMINOPURINE ON IN VITRO CLONAL PROPAGATION OF SOLANECIO B
- Author(s) : E. R. Ogbimi, A. M. A. Sakpere and B. E. Ayisire