PAPER TITLE :PRODUCTION AND PURIFICATION OF ? - AMYLASE FROM THE MUSHROOM, PLEUROTUS OSTREATUS.

APPLIED TROPICAL AGRICULTURE | VOLUME 15 NUMBER 1 2010

Paper Details

  • Author(s) : B. J. AKINYELE, D. J. AROTUPIN AND Y. D. FAGBOHUNGBE
  • Abstract:

Production of â-amylase from edible mushroom Pleurotus ostreatus was carried out using agricultural wastes namely,
sawdust, rice bran, wheat bran and palm kernel pericarp as substrates. â-amylase isolated from Pleurotus ostreatus
was purified by ion exchange chromatography on DEAE Sephadex A 50 and Gel filteration. The effect of variation in
pH, temperature and different salts on the activity of the enzyme were investigated. The optimum pH and temperature
was recorded to be 5.5 and 550C respectively. The Michaelis-Menten constant (Km), was 3.57umol/min/ml while the
maximum velocity (Vmax), was 0.21 μmole/min/ml. The effect of salts showed Hg Cl to be the most potent inhibitor
and CaCl the least. â-amylase from Pleurotus ostreatus could favorably be substituted for the ones obtained from
other sources since they are becoming too expensive for industrial applications owing to the global economic meltdown.