FUTA JOURNAL OF RESEARCH IN SCIENCE

VOLUME 13 NUMBER 2 2017

Paper Details

  • Title :SEROPREVALENCE AND GENOTYPING OF HEPATITIS B VIRUS INFECTION AMONG PATIENTS ATTENDING SELECTED HOSPITALS IN AKURE, NIGERIA.
  • Author(s) : *Onifade, A. K and Otun, K. O.
  • Abstract:

This study was carried out to determine the seroprevalence of Hepatitis B virus (HBV) infection in relation to socio-demographic and associated risk factors among hospital patients in Akure, Nigeria, and also to assess the molecular profile of HBV genotypes. A total of 724 blood samples were collected from 296 males and 428 female patients attending some selected hospitals in Akure from November, 2015 to January, 2016. Questionnaires containing relevant information about the respondents were administered appropriately to the female patients. Hepatitis B surface antigen (HBsAg) was assayed using ABON hepatitis B surface antigen (HBsAg) serum/plasma test strip kits. The positive serum samples were subjected to molecular analysis by Polymerase Chain Reaction (PCR) assay using genotype specific primers. The detection of HBV Deoxyribonucleic acid (DNA) was performed using multiplex PCR. Of the 724 samples screened for the study, 12(1.7%) were positive for HBsAg. Males were found to be more infected (2.7%, 8/296) than females (0.9%, 4/428) with no significant difference. The age group 18-28years had the highest prevalence rate (2.7 %, 4/146), while the lowest prevalence rate (1.8 %, 3/169) was observed in the age range 40-50 years. No significant difference was observed in the seroprevalence of HBsAg among the different age groups. Moreover, of the 12 HBsAg positive serum samples, only 3 samples were HBV DNA positive, of which they were all genotype E. It is hoped that findings from this study will be useful for health providers and policy makers in this area to design effective preventive programs and strategies against this viral infection.
Keywords: Prevalence, Genotype, Hepatitis B Virus, Antigen, Antibody