PAPER TITLE :HAEMATOLOGICAL AND SERUM BIOCHEMICAL PROFILES OF CLARIAS GARIEPINUS JUVENILES FED DIETS CONTAINING DIFFERENT INCLUSION LEVELS OF MECHANICALLY EXTRACTED SUNFLOWER (HELIANTHUS ANNUUS) SEED MEAL

APPLIED TROPICAL AGRICULTURE | VOLUME 22 NUMBER 2 2017

Paper Details

  • Author(s) : Adesina, S.A.
  • Abstract:

A fifteen-week experiment was conducted to evaluate changes in the blood indices of Clarias gariepinus juveniles fed with
six isonitrogenous and isocaloric diets in which mechanically extracted sunflower seed meal (MESSM) was substituted for
soybean meal at 0, 20, 40, 60, 80 and 100%. A total of 360 juveniles of C. gariepinus (mean weight 21.3 ± 0.1 g) were
randomly allocated into eighteen rectangular tanks (60 cm x 45 cm x 30 cm) at 20 fish per tank and fed twice daily at 5%
body weight. Fish blood samples were collected to determine blood indices. The results showed that fish fed 20% MESSMbased
diet had the best weight gain (51.65 g) while those on 100% MESSM-based diet had the least weight gain (21.09 g).
There were elevated values of PCV (28.67-38.00%), Hb (9.60-12.70 gm/100ml), RBCs (8.11-10.21 x 1012/mL), WBCs (8.33
- 12.53 x109/mL), platelets (10.00-14.00 x109/mL) and lymphocytes (57.67-75.00%). Fish fed 20% MESSM inclusion had
the highest PCV (38.0% ), Hb (12.70 gm/100ml), RBC (10.21 x 1012/mL), platelets (14.00 x109/mL), MCV (37.00 μg/ml)
and neutrophils (42.33%). Fish fed 40% MESSM had the highest total protein (4.17 g/100 mL), globulin (3.10 g/100 mL),
K+ (45.67 mg/dl), Na+ (85.67 mg/dl), creatinine (2.24 mg/dl), alanine aminotransferase (ALT, 37.67 mg/dl) and aspartate
aminotransferase (AST, 51.33 mg/dl). Significant difference (p<0.05) existed among the values obtained for the various
parameters examined. Blood glucose significantly (p < 0.05) increased (62.33 - 81.33 mg/dl) in fish fed 20 - 100% MESSM.
This study revealed that mechanically extracted sunflower seed meal can replace soybean meal in the diet of C. gariepinus
without any adverse effect on its blood parameters.
Key words: Blood samples, Clarias gariepinus, Haematology, Soybean meal, Sunflower seed meal