PAPER TITLE :ASSESSMENT OF THE GENETIC VARIATION IN CHRYSOPHYLLUM ALBIDUM G. DON USING CP SSR MARKER
APPLIED TROPICAL AGRICULTURE | VOLUME 23 NUMBER 1 2018
- Author(s) : Boboye, O. M., Lawal, A. and Oyerinde, O. V.
Chrysophyllum albidum is among the forest tree species that provides Non-Timber Forest Products (NTFPs) of enormous domestic significant to both rural and urban dwellers in West Africa, with great export potentials. The fruit pulp is widely consumed and thus play a significant role in food security. C. albidum, which is classified among the endangered tree species with a high probability of going into extinction in the nearest future, is endowed with a high degree of climatic adaptability. This indicate that there may exist a tremendous genetic variation that needs to be identified for immediate conservation. In this study, total genomic DNA was extracted from 0.020 g powder of silica gel dried leaves using the QIAGEN DNeasy plant Mini Kit according to the manufacturer’s instruction. DNA was amplified by Polymerase Chain Reaction (PCR). The estimation of total gene diversity (Ht), gene diversity due to variation within population (Hs), gene diversity due to differences among populations (Gst) and gene flow (Nm*) were 0.1330±0.041, 0.0472±0.002, 0.645, and 0.275 in that order. This reveals that genetic base of this important tree species has been seriously eroded. Therefore, urgent steps must be taken by individual, organizations and government to conserve the genetic base of this important tree species. Key words: Chrysophyllum albidum¸ conservation, genetic variation, microsatellites